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How long must universal holders be placed in an autoclave at 134 degrees Celsius for effective sterilization?

  1. 2 minutes

  2. 4 minutes

  3. 8 minutes

  4. 10 minutes

The correct answer is: 4 minutes

The correct duration for universal holders to be placed in an autoclave at 134 degrees Celsius is 4 minutes. This specific time frame is established based on guidelines for effective sterilization processes, particularly when dealing with porous and non-porous materials, such as those made for microblading tools. At 134 degrees Celsius, the pressurized steam penetrates and disinfects the items thoroughly within this period, ensuring that harmful microorganisms, including bacteria and viruses, are effectively eliminated. The effectiveness of sterilization not only relies on the temperature but also significantly on the exposure time within the autoclave to ensure comprehensive coverage and treatment of all surfaces. This 4-minute benchmark is vital for practitioners to maintain safety and hygiene standards, ensuring the tools used for microblading are properly sanitized before each use. Understanding the significance of this timing helps in emphasizing proper sterilization practices in the industry.